Department of Natural Sciences

Forensic Biology

Lecture

Application of forensic DNA profiling; identifying biological evidence;
DNA isolation methods; short history of forensic biology; STR systems
and their analysis via multiplex PCR and capillary electrophoresis;
population genetics, databases and calculation of random match
probabilities; qPCR; profiling of mtDNA; paternity testing; Y-STRs;
forensic species determination (Cytb, COI, STRs)

→ Download scripts, group assignment etc. (LEA)

Practical Course

DNA isolation from blood and buccal swabs; DNA quantitation using realtime
PCR; STR analysis using multiplexPCR and capillary electrophoresis; mtDNA-based
determination of meat species; forensic sex typing (amelogenin)

→ Download scripts, group assignment etc. (LEA)

Requirements

Enrolment in the 3. semester of the programme "Bachelor of Science in Naturwissenschaftlicher Forensik" at the University of Applied Sciences Bonn-Rhein-Sieg.
Successful attendance of the Special Safety Instructions given in the 2. semester (see Fundamental Biology-Course).

Assessment:

Written exam – graded
Successful participation in the laboratory course , documented by a laboratory report, is a prerequisite for passing the final examination.

Literature

John M. Butler: Fundamentals of Forensic DNA Typing (Elsevier)
William Goodwin, Adrian Linacre, Sibte Hadi: An Introduction to Forensic Genetics, 2nd Edition (Wiley-Blackwell)