Fachbereich Angewandte Naturwissenschaften

Methods of Bioanalysis and Laboratory Diagnostics (MSc)

Vorlesung

Es handelt sich um ein Pflichtmodul 

  • für Studierende des 2. Sem. Analytische Chemie und Qualitätssicherung
  • Angebot jeweils im Sommersemester
  • Umfang: 6 Semesterwochenstunden (3V/1Ü/2P)
  • Credits: 8 ECTS

 

Vorlesung und Übung

  • General introduction and safety instructions
  • Proteins: Quantitation, Purification and Structural analysis (including IEF, chromatography, ELISA)
  • MS and MS-based approaches
  • DNA: DNA structure, purification, PCR
  • DNA: Sanger sequencing, capillary electrophoresis, DNA microarrays, NGS
  • Urinary organic acids and the human metabolome
  • Laboratory diagnostics with a focus on clinical chemistry
  • Quality assurance in the medical diagnostic lab (Rili-BÄK)
  • Excursion to a diagnostic laboratory (Newborn screening for metabolic and endocrine diseases; selective screening-Analysis of glycans and lipids)

 

Internship

Praktikum
Anwesenheitspflicht. Proteinquantifizierung, gekoppelter
Enzymaktivitätstest, SDS-PAGE und Western Blot, Real Time PCR,
Nachweis einer Sequenzvariante; verschiedene Verfahren der
Auftrennung von Nukleinsäuren.

Teilnahmevoraussetzungen

Basic education in (analytical) chemistry and biology. Participation in the practical course requires prior participation in the safety instructions. Students are only allowed to join the practical course if they are familiar with the course material.

The result of a written exam, which is based on lecture, exercise and practical class, will yield the final mark. Active full-time participation in all four events of the practical course is also a requirement for passing the module.

Note: The course comprises an excursion to a diagnostic laboratory.

Literatur

  • Campbell & Farell, Biochemistry, Cengage Learning , 8th ed., 2015
  • Lottspeich & Engels, Bioanalytik, Springer-Spektrum,  3rd ed., 2012
  • Manz et al., Bioanalytical Chemistry, Imperial College Press, 2nd ed., 2015

Sie haben noch Fragen?

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Jörn Oliver Sass

Professor für Bioanalytik und Biochemie, Forschungsgruppe Angeborene Stoffwechselstörungen (FG Sass)

joern.oliver.sass@h-brs.de

Standort

Rheinbach

Raum

I 218; Labor F 012

Adresse

von-Liebig-Str. 20

53359 Rheinbach

Telefon

+49 2241 865 9668
Prof. Dr. Jörn Oliver Sass

Dokumente

zum Herunterladen

Modulhandbuch M.Sc. Analytische Chemie und Qualitätssicherung (2022) (.pdf (DE))